ABSTRACT
The present work takes into account the development of Reverse Phase High Performance Liquid Chromatography(HPLC) for simultaneous method estimation and validation of pyrimethamine and sulfamethoxypyrazine inpharmaceutical formulation. The chromatographic separation was accomplished on C8 column by using acetonitrileand potassium dihydrogen phosphate as the mobile phase (60:40 v/v) having a flow rate of 0.8 ml/minute. Theeluent was detected at 254 nm, simultaneously for both the drugs. The retention time for pyrimethamine andsulfamethoxypyrazine was found to be 3.33 and 4.21 minutes, respectively. According to the International Conferenceon Harmonisation guidelines, the develop method was validated in terms of accuracy, precision, linearity, limit ofdetection, limit of quantitation, robustness, and stress degradation studies. This validated method can be suggested forthe routine simultaneous laboratory analysis of pyrimethamine and sulfamethoxypyrazine.
ABSTRACT
Lidocaine (LDC) and prilocaine (PLC) are estimated in a topical local anesthetic cream using a direct, eco-friendly,stability-indicating gas chromatographic technique with flame ionization detector. The mixture of LDC and PLCwas separated using Zebron DB drug column. The column temperature and flow rate were 230°C and 14 ml/minute,respectively. The retention time was found to be 5.1 minutes for PLC and 5.4 minutes for LDC. Linearity was observedin the concentration range of 20–100 μg/ml and 10–50 μg/ml for LDC and PLC, respectively. The method was validatedand values of linearity, limit of detection, limit of quantification, precision, and accuracy were found to be in goodaccordance with the International Conference on Harmonization guideline. A direct, stability-indicating method wasdeveloped for the determination of LDC and PLC in topical dosage forms in the presence of its degradation products.The proposed method can be useful in the quality control of LDC and PLC in their topical formulation.